The Evaluation of Proton Pump Inhibitor Bioactive Fraction DLBS2411 from Cinnamomum burmannii (Nees & T. Nees) in Animal Model of Gastric Ulceration Healing

Abstract

DLBS2411 is a bioactive fraction from Cinnamomum burmannii that is believed to possess anti-ulceration activity. In this study acetic acid was administered to induce gastric ulcer. Rats were divided into six groups: Healthy animals, untreated animals (acetic acid-induced), treatment group of BAF DLBS2411 25 mg kg^-1 Body Weight (BW), BAF DLBS2411 50 mg kg^-1 BW and positive control group of omeprazole 2 mg kg^-1 and sucralfate 100 mg kg^-1 BW. The efficacy of DLBS2411 was measured through histopathology examination and ulceration observation. Hematological parameters were also evaluated before and after treatments to indicate the safety profile of DLBS2411. The untreated animals developed more serious injuries (i.e., bigger and more severe ulcers) than the positive control groups and DLBS2411 treatment groups. DLBS2411 treatment with doses of 25 and 50 mg kg^-1 and sucralfate 100 mg kg^-1 were effective to heal the ulcer as shown by reduction of ulcer size (up to 54.8, 36.53 and 14.85%, respectively) compared to the untreated animals. DLBS2411 was as effective as omeprazole 2 mg kg^-1 and sucralfate 100 mg kg^-1 in alleviating ulcer severity as examined in histopathology result. There were no significant differences in hematological values. DLBS2411 at doses of 25 and 50 mg kg^-1 demonstrated healing activity on acetic acid-induced gastric ulcer, with a similar effect to that of omeprazole at dose of 2 mg kg^-1 or sucralfate at dose of 100 mg kg^-1. No adverse reaction was seen in all treatment groups, suggesting that DLBS2411 has a good safety profile in animal.