Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme
- 1 Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No. 3, Bulaksumur, Yogyakarta, Indonesia
- 2 Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No. 3, Bulaksumur, Yogyakarta, India
- 3 Halal Materials Development, Institute for Halal Industry & System, Gadjah Mada University, LPPT Building, Jl. Kaliurang Km 4.5, Yogyakarta, Indonesia
Abstract
The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis (SDS-PAGE). The results showed the highestcollagen yield was 7.77% from D treatment; the viscosity ranges were 2.18-1.99cP, and the pH ranges were 4.89-4.68. FTIR spectra showed amide A, B, I, II,and III. SDS-PAGE results showed three distinct main bands, consisting of α1,α2, and β chains. In conclusion, collagen could be extracted from Local duckfeet and categorized as collagen type I.
DOI: https://doi.org/10.3844/ajavsp.2025.97.102
Copyright: © 2025 Rafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin and Yuny Erwanto. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Duck Feet
- Collagen Isolation
- Bromelain Enzyme
- Characterization