Follicle Stimulating Hormone and Gonadotropin Releasing Hormone Administration to the Superovulation of Buffalo (Bubalus bubalis)

Abstract

This study aims to determine the response of superovulation, corpus luteum number, onset and duration of estrus in Swamp Buffalo after administration of Follicle Stimulating Hormone (FSH) and Gonadotropin Releasing Hormone (GnRH). The material used is 16 mother Swamp Buffaloes with an average body weight of 500 kg and age between 3-5 years. The dairy performance index such as the lactation period was 127.5±104.6 days, the milk production accounted for 2.0±0.9 kg/cow/day and the milk yield was 255±209 kg/lactation. The experimental design used was a Completely Randomized Design (CRD) consisting of four replications. Swamp buffaloes are synchronized with 5 mL of PGF2α injection. GnRH hormone injection on the 8th day at a dose of 500 µg with 2 times in the morning and evening. FSH injection begins on the 10th day with 2 times a day i.e., morning and night (12 h interval) with a dose decreased for 3 days intra-muscularly. Artificial insemination was carried out after signs appear estrus. The parameters observed include the superovulation response, the number of corpus luteum, the onset and duration of estrus in the Swamp Buffalo. The results obtained are the superovulation response in 56.25% Swamp Buffalo. The average number of corpus luteum, speed and duration of swamp buffalo estrus at different FSH doses and addition of 500 μg GnRH were 1.75, 20.25 and 145.75 h respectively. It was concluded that superovulation by administering a dose of 16 ml FSH + 500 μg GnRH has shown the best superovulation response and a long duration of estrus. The administration of 22 mL FSH dose and the addition of 500 μg GnRH showed the fastest onset of estrus.