Seedling Propagation of Kulo Chrysanthemum by Tissue Culture

Abstract

Aims of this research were to get the right dosage of Plant Growth Regulkator (PGR) and Coconut Water (CW) for shoot multiplication of Kulo chrysanthemums. The study was conducted using a factorial design of three factors namely IAA (0.0; 0.5 and 1.0 ppm), BAP (0, 0.5 and 1.0 ppm) and coconut water (0.0 and 30%). The explants used were clean micro cuttings and were cultured on Murashige and Skoog (MS) media. The media was added IAA, BAP and coconut water. The result showed that the best shoot multiplication of Kulo chrysanthemums was the MS media using combination of 0.5 ppm and 1.0 of IAA, 0.5 ppm of BAP and 30% of coconut water. Good rooting was revealed by the combination of IAA 0.5 and 1 ppm with 30% coconut water and BAP 1 ppm and 30% coconut water.